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Accueil > La recherche > Département Bioingénierie > Priorité T2 : Biomicrosystèmes, Bioélectrochimie et Bioélectromagnétisme > Axe Microsystèmes

Electrorotation

par Naoufel - publié le , mis à jour le

Electrorotation is a technique consisting in subjecting a polarisable particle to a uniform rotating electric field, causing it to spin. This tool enables single cell characterization and allows the measurement of membrane and cytoplasm electrical properties. The permittivity and conductivity of these cellular components may be determined by placing the cell in a rotating electric field generated by microelectrodes.
The induced cell rotation rate depends on the cellular properties and on the applied electric field frequency. We have developed an automated experimental platform (field generation, acquisition and image processing, extraction of cell properties) allowing to access the electrical properties of cells (yeast, bacteria, eukaryotes) and cell models (liposomes) thanks to their electrorotation spectra.
These experimental data provide an electrical model of cells for numerical studies of electromagnetic field / cell interaction and can also be used to to detect changes in the physiological state of single cells after a specific treatment.


Related publications

- El-Gaddar A., Frénéa-Robin M., Voyer D., Aka H., Haddour N., Krahenbuhl L. « Assessment of 0.5 T static field exposure effect on yeast and HEK cells using electrorotation », Biophys J. 2013 Apr 16 ;104(8):1805-11

- Voyer D., Frénéa-Robin M., Buret F., and Nicolas L., "Improvements in the extraction of cell electric properties from their electrorotation spectrum," Bioelectrochemistry 2010 79(1) : 25-30

- Laforêt J., Frénéa-Robin M., Buret F., Nicolas L., “An automated platform for cell dielectric spectroscopy”, International Journal of Bioelectromagnetism, n°1, vol.9, 2007, 26-27.