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Home > Research > Bioelectromagnetism and Microsystems > Microsystems

Electrochemical characterization

by Naoufel HADDOUR - published on , updated on

We study permeabilization of cell membanes exposed to electric field pulses in order to better understand the electropermeabilization process and to handle it for medical and environmental applications.

At the moment, studies are in their infancy and require the use of biological models that can be simple to isolate, monitor, and characterize experimentally.
Our group uses liposomes that mimic the membrane of biological cells. Liposomes are synthesized in a controlled way and offer the possibility of changing experimental parameters such as size of membranes, their composition and the composition of internal and external environment of the membrane and to study their effect on the electropermeabilization process.

Schematic representation of the electrochemical characterization method employed to study electroporation of a liposome encapsulating redox markers.

We study electrochemically the dynamics of electroporation of liposomes enclosing electroactive molecules. The electrochemical signal (current oxidation or reduction) of electroactive molecules released after exposure of liposomes to electrical field pulses allows to monitor the dynamics of electroporation. The amount of electroactive molecules released is determined by electrochemical measurements and depends on the permeabilization rate of studied liposomes. This permeabilization rate varies with the applied electric fields and membrane composition.